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WBPicture0000014488Description(A) Rescue ability of varioustransgenes expressing GFP-taggedLPR-1 with or without a signal sequence. Bars indicate percentageof transgenic (Tg) (black) or nontransgenic (non-Tg) (white) animals showing normal excretorytube morphology at the L1 stageas assessed by DIC microscopy.***P , 0.0001 compared to nontransgenic siblings (Fisher's exacttest). (B and B') Expression patternof lpr-1p::LPR-1::SfGFP at 1.5-fold.Duct and pore cells are marked bygrl-2p::mRFP (red). (C and C') Expression pattern of lpr-1p::ssGFP::LPR-1(25-261) at 1.5-fold. Boxesin (B) and (C) indicate the regionsmagnified in (B') and (C'). (D, D',D'', and D''') Expression pattern oflpr-1p::LPR-1::SfGFP at threefold.(D'') and (D''') show a confocalprojection of the duct and porefrom the same embryo. White arrows indicate fusion protein accumulating in the space betweenthe embryo and the eggshell. (Eand E9) Duct and pore cells aremarked by grl-2p::mRFP. (F and F') lpr-1p::ssGFP::LPR-1(25-261)was expressed in the epidermisand accumulated in the spacebetween the embryo and theeggshell, indicated by white arrows. In an early threefold embryo,lpr-1p::ssSfGFP::LPR-1(250-261) wasenriched cortically in the duct andpore (marked in red).
NameFig2_A.jpg
CropCropped_fromWBPicture0000014487
DepictExpr_patternExpr13230
AnatomyWBbt:0004540
WBbt:0005778
Cellular_componentGO:0005576
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2016
Article_URLDOIid10.1534/genetics.116.195156
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00050556