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| WBPicture0000014362 | Description | S2 Fig. let-653 is expressed in external epithelia.Additional data related to Fig 5. (A, B) let-653pro::GFP reporter sEx10642 expression in the major hypodermis (hyp) at ventral enclosure (A) and in the excretory duct and pore at the 3-fold stage (B). Expression is only occasionally and transiently observed in the canalcell. A' and B' show DIC images for comparison. (E) LET-653b::SfGFP driven by the canal-specific glt-3 promoter occasionally accumulates in the duct lumen of embryos. (F) LET-653b::SfGFP driven by the non-cycling lin-48 promoter also shows an oscillatory pattern of accumulation in the duct lumen. n>15 animals for each timepoint. (G-L) Both let-653pro::LET-653::SfGFP and let-653pro::ssSfGFP::LET-653 reveal apical secretion and accumulation in extracellular and luminal regions. (G, J) ventral enclosure embryos.(H, K) 3-fold embryos. E',G',H',I',J' show merged DIC images for comparison. (I, L) Both LET-653::SfGFP and ssSfGFP::LET-653 are absent from the early L1 duct cell lumen, after mature cuticle secretion and hatch. K' and L' show absent SfGFP signal. ssSfGFP::LET-653 appears within the space between the old and new cuticles during the L1/L2 molt (M,N). M' and N' show DIC images for comparison. Scale bars, 10 um. | |||
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| Name | FigS2.jpg | ||||
| Depict | Expr_pattern | Expr13008 | |||
| Anatomy | WBbt:0004432 | ||||
| WBbt:0004534 | |||||
| WBbt:0004540 | |||||
| WBbt:0005733 | |||||
| WBbt:0005812 | |||||
| WBbt:0008182 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2016 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1006205 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00049934 |
