WormBase Tree Display for Picture: WBPicture0000014278

WBPicture0000014278 Description: Figure S2. The csr-1(tm892) Mutant, GFP::KBP-4 Localization in csr-1(RNAi), the gfp::csr-1 Transgene Insertion, and GFP::CSR-1 Localizationin the Absence of Endogenous CSR-1, Related to Figure 2(A) The csr-1(tm892) mutant. The 400 bp deletion in tm892 removes the indicated intronic and exonic sequences and introduces a frameshift mutation after residue number 44 of CSR-1b (which is 847 aa long), resulting in encoding of 13 additional out-of-frame amino acids followed by a stop codon.(B) Line scan analysis of GFP::KBP-4 in control and csr-1(RNAi). KBP-4 is a subunit of the microtubule-binding NDC-80 complex. n is the number of chromosomes analyzed. For representative images, see Figure 2F.(C) Schematic of the gfp::csr-1 transgene inserted in single copy at a Mos transposon site on Chr II. The coding sequence for GFP was inserted at a position that results in translation of GFP after aa 5 (encoded by exon 1) of CSR-1b and after aa 168 (encoded by exon 2) of CSR-1a. The transgene was also recoded for RNAi resistance as described in Figure S3A.(D) The gfp::csr-1 transgene rescues the embryonic lethality and sterility of csr-1(tm892). Embryo viability and brood size was measured for the indicatedconditions. For csr-1(tm892), first generation homozygous worms derived from balanced heterozygous mothers were analyzed. Error bars are the SD.(E) Localization of GFP::CSR-1 in early embryos (left panel set) and in the larval and adult germline (right panel set). P-granule localization is highlighted by green arrows in the embryo posterior (left panels) and in the perinuclear region in the germline (green arrows). The blowup of the L4 larva germline image (yellow box) shows the clustered perinuclear signal. Blowups of two oocyte nuclei in the adult germline highlight the GFP::CSR-1 signal in the nuclear periphery and absence of detectable chromosome enrichment. Scale bars 10 um in all images except magnified region of L4 larva germline, where scale bar is 5 um.
  Name: FigS2.jpg
  Depict: Expr_pattern: Expr12894
    Cellular_component: GO:0043186
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2016
    Article_URL: DOI id 10.1016/j.cell.2016.02.040
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00049397