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| WBPicture0000014266 | Description | Figure 2. VPS-50 Functions in Neurons(A) Fluorescence and Nomarski micrographs of regions of C. elegans transgenic animals expressing VPS-50::GFP (nIs388) using the vps-50 promoter. vps-50 was expressed in most, if not all, neurons. Occasional expression was observed in some pharyngeal muscles. VNC, ventral nerve cord. #To enhance visualization,we used unc-104(e1265) mutant animals to increase the fluorescence level in cell bodies. Scale bars, 10 μm.(B) vps-50 functions in neurons to regulate locomotion. vps-50 was expressed pan-neuronally (Prab-3) and in body-wall muscles (Pmyo-3) to identify its site ofaction. Pan-neuronal expression of vps-50, or of its murine homolog mVps50, rescued the locomotion defect of vps-50(n4022) animals. n = 3 plates for allgenotypes.(C) vps-50 functions in cholinergic neurons to regulate locomotion on food. vps-50 was expressed in cholinergic neurons (Punc-17), in GABAergic neurons(Punc-47), and in a subset of sensory neurons (Ptax-2) to identify its site of action. n = 5 plates for the wild-type and vps-50(n4022); n = 3 plates for all othergenotypes.(B and C) Significance is defined by comparison to the equivalent state for vps-50(n4022) mutants. Means ± SDs.(D) The localization of VPS-50::GFP to synapse-rich areas of the nerve ring depends on UNC-104/KIF1A, a molecular motor that transports synaptic vesicles andtheir associated proteins to synapses. Immunohistochemistry against GFP and synaptobrevin (SNB-1) and DAPI staining are shown. The dotted lines indicate thesynapse-rich nerve ring, and clusters of neuronal cell bodies are marked. Scale bar, 10 μm. | |||
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| Name | FigB.jpg | ||||
| Crop | Crop_picture | WBPicture0000014267 | |||
| Depict | Expr_pattern | Expr12790 | |||
| Anatomy | WBbt:0005451 | ||||
| WBbt:0005735 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2016 | ||||
| Article_URL | DOI | id | 10.1016/j.cub.2016.01.049 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00049339 |
