WormBase Tree Display for Picture: WBPicture0000014098

WBPicture0000014098 Description: FIGURE 1: Promoter analysis of unc-87A and unc-87B. (A-N) Promoter activity of unc-87A (A-H) and unc-87B (I-N) was examined using GFP as a reporter. Each fluorescence micrograph of GFP (black and white) is paired with a differential interference contrast image overlaid with green fluorescence of GFP. Images of whole worms with their heads on the left (A, B, I, and J) and magnified views of representative tissues (C-H, K-N) are shown. Note that the micrograph in C is somewhat overexposed for GFP in the pharynx to demonstrate relatively weak GFP in the neurons. (O-W) Expression ofGFP in hermaphrodite gonads with no transgene (O-Q), Punc-87A::GFP (R-T), or Punc-87B::GFP (U-W) was examined by immunostaining of dissected gonads for GFP (O, R, and U) and MYO-3 as a marker of myoepithelial sheath (P, S, andV). Merged images are shown in Q, T, and W (green, GFP; red, MYO-3; blue, DNA [not shown as individual images]). Scale bars, 100 um (whole worms; A, B, I, and J), 20 um (magnified tissues; C-H,K-N), 50 um (dissected gonads; O-W).
  Name: Fig1.jpg
  Crop: Crop_picture: WBPicture0000014099
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  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2015
    Article_URL: DOI id 10.1091/mbc.E14-10-1483
    Journal_URL: MolecularBiologyoftheCell
    Publisher_URL: TheAmericanSocietyForCellBiology
  Reference: WBPaper00046489