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| WBPicture0000013140 | Description | Figure 2. epg-7 encodes a protein with similarity to mammalian FIP200 and yeast Atg11 and is degraded by autophagy. (A) Cloning of epg-7. (B) Protein sequence of EPG-7. The molecular lesions in identified mutant alleles are highlighted in red. (C) Schematic structures of EPG-7, FIP200, and Atg11. The coiled-coil domains are highlighted in green. The Atg11 domain is depicted in pink. (D) A large number of SQST-1::GFP aggregates accumulate in epg-7 mutant embryos carrying an epg-7(del Atg11) transgene. (E and F) No SQST-1 aggregates are detected in epg-7 mutants carrying an epg-7::gfp reporter. (E) DAPI image of the embryo shown in F. (G) EPG-7::GFP is weakly expressed and diffusely localized in the cytoplasm in wild-type embryos. (H-I) EPG-7::GFP is expressed in pharyngeal muscles (H, arrow) and cells in the tail region (I) at larval stages. (J) Compared with wild-type animals, levels of EPG-7::GFP are dramatically elevated in epg-4 mutants, but remain unchanged in sqst-1 mutants. (K) The expression level of EPG-7::GFP is dramatically elevated and many EPG-7::GFP aggregates are formed in lgg-1 mutant embryos. (L and M) No EPG-7 aggregates, detected by anti-EPG-7 antibody, are formed in wild-type embryos. (L) DAPI image of the embryo shown in M. (N) Levels of EPG-7 are dramatically elevated and a large number of EPG-7 aggregates are formed in atg-3 mutants. (O) No EPG-7 aggregates are formed in sqst-1 mutant embryos. Bars: (D-G and K-O) 10 um; (H) 10 um; (I) 10 um. | |
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| Name | F2.large.jpg | ||
| Crop | Crop_picture | WBPicture0000013141 | |
| Acknowledgment (5) | |||
| Reference | WBPaper00042170 |
