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WBPicture0000013132DescriptionFigure 4. csp-1 is expressed in late pachytene cells of the L4 and adult hermaphrodite germline. Fluorescence in situ hybridization images of gonad arms of (A) an L4 hermaphrodite and (B) an adult hermaphrodite hybridized with Cy5-labelled probes complementary to csp-1B. The Cy5-labelled probes are visible as green puncta; the gonads are outlined in white. A schematic representation is shown above each micrograph. (C) Fluorescence in situ hybridization images of an adult hermaphrodite gonad hybridized with ALEXA594-labelled probes (red puncta) complementary to the region of csp-1A that encodes the prodomain (csp-1A) and Cy5-labelled csp-1B probes (green puncta) that hybridize to all csp-1 transcript isoforms (total csp-1). White arrowheads indicate csp-1A-specific puncta; orange arrows indicate csp-1B-specific puncta, which are recognized strongly by the total csp-1 probes but only weakly by the csp-1A-specific probes. (D) The number of CED-1::GFP-positive apoptotic cells in the gonads of caspase mutants exposed to 0 Gy and 120 Gy of ionizing radiation at the L4 larval stage. The strains were scored at 24 hrs and 48 hrs post L4-stage. Error bars indicate standard deviations.
NameFigD.jpg
CropCrop_pictureWBPicture0000013133
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2013
Article_URLDOIid10.1371/journal.pgen.1003341
Journal_URLPLoSGenetics
Publisher_URLPLoS
ReferenceWBPaper00042142