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| WBPicture0000010964 | Description | Figure 6. Expression of PLX-2 and EFN-4 in embryonic neuronal and epidermal cells. PLX-2 and EFN-4 GFP transgene expression was detected by immunostaining with anti-GFP antibodies (green in all panels) ; samples are labeled with the anti-AJM-1 antibody MH27 (red) to visualize epidermal cell outlines. A and B are stained with DAPI (blue) to show cell nuclei. (A) A PLX-2::GFP translational fusion protein is first expressed in a small number of ventral cells prior to epidermal enclosure; these are identified as ventral neuroblasts on the basis of position. (B) In late stage embryos, PLX-2::GFP was expressed in several tail epidermal cells, including the preanal ventral epidermal cell pairs P11/12 and P9/10 (indicated as 'P' with white lines). PLX-2::GFP was also expressed in numerous neurons in the head and tail. (C and D) Expression of PLX-2 in the QV5 lateral epidermal cell at the 1.5- and 2-fold stage, detected using the Pplx-2-GFP transcriptional reporter ncIs21; images are projections of surface focal planes from a confocal z-stack. (E-H) EFN-4::GFP (juIs109) is expressed in a subset of epidermal cells during and after epidermal enclosure. During enclosure (lateral views in E and F; ventral view in G), EFN-4::GFP was detected in the lateral epidermal cells H0 and QV5, in the leading anterior epidermal cells (green's') in the head region, and in the three posterior pairs of P cells (P7/8, 9/10, 11/12) (green P cells marked in AJM-1 channel in F). (H) At the 2-fold stage, EFN-4::GFP is seen in P9/10 and P11/12 (P, ventral view of preanal epidermis); expression also persists in the head ventral epidermis. EFN-4::GFP expression in epidermal cells persists until late embryonic stages and was not detectable in L1 larvae. Bar, 10 um in all panels except B (20 um). | |||
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| Name | F6.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000010965 | |||
| Acknowledgment | Template | WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL> | |||
| Publication_year | 2007 | ||||
| Article_URL | DOI | id | 10.1534/genetics.106.067116 | ||
| Journal_URL | Genetics | ||||
| Publisher_URL | GSA | ||||
| Reference | WBPaper00029396 |
