WormBase Tree Display for Picture: WBPicture0000010962

WBPicture0000010962 Description: Figure 8. VHA-5 and actin microfilament distributions are jointly reorganized during molting. Confocal micrographs showing the distributions of actin, as revealed by the vha-8::vab-10ABD::yfp construct noted ABD::YFP (it corresponds to the actin-binding domain of VAB-10 fused to YFP), VHA-5::CFP, and DLG-1::RFP transgenes at four different time points during larval development. (A) XY projections showing actin and VHA-5. Actin microfilaments form parallel bundles during molts (first and third rows), which become disorganized between molts (second and fourth rows). VHA-5 distribution (second column) follows the same pattern, forming aligned dots during molts and dispersed dots in intermolts. Both markers colocalized during molts (merge pictures). (B) Magnification of the apical side of the epidermis during the L4-adult molt when actin bundles are better organized. VHA-5::CFP dots (arrowheads) colocalize with ABD::YFP on the most apical and parallel actin bundles in both XY and XZ projections, rather than on the faint and less organized subapical actin filaments that we reproducibly observed in XZ projections (arrows). (C and D) ABD::YFP bundles surrounding the seam cells (arrowheads) colocalize with DLG-1::RFP at the apical junctions between the hyp7 syncytium (H) and seam cells (S). VHA-5::CFP is shown in violet in A and B (to enhance the contrast) and in blue in C and D.
  Name: F8.large.jpg
  Depict: Expr_pattern: Expr4813
    Anatomy: WBbt:0005733
  Acknowledgment: Template: WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
    Publication_year: 2007
    Article_URL: DOI id 10.1534/genetics.106.066035
    Journal_URL: Genetics
    Publisher_URL: GSA
  Reference: WBPaper00028924