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| WBPicture0000010732 | Description | Fig. 2. Sensitivity to anoxia results from loss of function of HYL-2 ceramide synthase. (A) GFP staining of hyl-2(gnv1) adult animals expressing the transgene GFP::hyl-2 under the control of the hyl-2 promoter. (B) Survival after 48 hours of anoxia of hyl-2(gnv1) animals complemented with the transgene GFP::hyl-2 under the control of the hyl-2 promoter (mean T SD, n > 5). (C) Functional complementation of mutant yeast cells by C. elegans hyl-1 and hyl-2 in lag1Dlac1D S. cerevisiae transformed with pRS424-hyl-1, pRS424-hyl-2, and pRS424-hyl-2(gnv1) (TRP1 plasmid). The compound 5-FOA (5-fluorouracil-6-carboxylic acid monohydrate) is a counterselection for cells that carry the URA3-based plasmid with yeast LAG1. Therefore, only cells that have lost this plasmid can grow, making growth dependent on function of the heterologous ceramide synthase. | |||
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| Name | F2.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000010733 | |||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 2009 | ||||
| Article_URL | DOI | id | 10.1126/science.1168532 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00033113 |
