WormBase Tree Display for Picture: WBPicture0000010632

WBPicture0000010632 Description: Fig. 2. The expression of the ltd-1 reporter in embryogenesis and during the post-embryonic development of Caenorhabditis elegans. The GFP expression (white or green signal) has been followed with confocal microscopy. The signal has been mainly observed in the hypodermis, which is involved in nematode elongation, through circumferential actin filament contraction and somatic cell fusion (Chin-Sang and Chisholm, 2000; Sulston and Horvitz, 1977). (A) Lateral view of a twofold stage embryo. Anterior is to the right. The arrow points to the right lateral seam cells expressing the reporter gene. The fluorescent signal is also observed in the ventral and dorsal hypodermal cells as tight circumferential bundles (triangles). (B) End of embryogenesis. Anterior is to the left. The arrows point to the right row of seam cells. Note the patch of intense signal at both extremities of seam cells (triangles). (C) Higher magnification of the anterior part of an L1 larva. Anterior is to the left. The arrows point to the seam cells. The signal is evident in the junctions between hypodermal cells hyp 5, hyp 6 and hyp 7. (D) Anterior is to the right. The right row of seam cells in an L1 larva. The arrow points to an anterior daughter seam cell. (E) Higher magnification of an L1 larva. The signal is particularly intense at the junction between the different hypodermal cells. The triangle indicates the junction between two ventral midline P cells. Note the circumferentially oriented bundles in the ventral hypodermis (arrows). (F) Anterior is to the right. Fluorescence is clearly seen at the apical junctions between ventral hypodermal cells (triangles). (G) Molting L2 larvae. The arrow points to one daughter seam cell, which is progressively sinking into the hyp 7 syncitium. Note the very intense GFP signal at the junction between the elongating seam cells. (H) This is a non-molting L2 larvae. Note the absence of signal in two of the five seam cells shown (arrow). This difference in signal intensity could be attributed to differing stages in the molting process among the seam cells. (I) Anterior is to the right. Higher magnification of an L4 larva illustrating an intense longitudinal signal in the cytoplasm of a seam cell (arrows). (J) In the adult stage, the signal is located in the alae formed by the seam cells after fusion (arrow). (K) Anterior is to the right, and dorsal to the top. L2 larva, showing GFP expression in the Y and U rectal epithelial cells (arrows). The triangle points to the apical pole of both cells lining the animal's rectum. (L) ltd-1 reporter expression in the pharynx and socket cells of an L3 larva. The signal is visible in the terminal bulb in what have been tentatively identified as marginal cells (thick arrow) and at the junction between the pharynx lumen and the hypodermis (triangle). GFP signal is also observed in the processes that socket cells send to the amphid (arrows). All bars represent 10 um.
  Name: F2.jpg
  Depict: Expr_pattern: Expr2252
    Anatomy: WBbt:0003673
      WBbt:0003681
      WBbt:0004578
      WBbt:0004679
      WBbt:0004685
      WBbt:0004942
      WBbt:0005733
      WBbt:0005734
      WBbt:0005750
      WBbt:0005753
    Cellular_component: GO:0005737
      GO:0005911
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1016/S0925-4773(02)00182-X
    Journal_URL: MechanismsofDevelopment
    Publisher_URL: Elsevier
  Reference: WBPaper00005491