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WBPicture0000008937DescriptionFigure 1. CeCENP-A and CeCENP-C colocalize to the diffuse kinetochores of one-cell stage C. elegans embryos. (A) Wild-type embryos were stained to visualize DNA (cyan), MTs (red), Ce-CENP-A, and CeCENP-C. Different stages during the first division of the C. elegans embryo are shown. The sperm-derived pronucleus with associated centrosomal asters is to the right and the oocyte-derived pronucleus to the left in the early prophase and prophase panels. The weak spindle pole staining in the Ce-CENP-A metaphase and anaphase panels is nonspecific. (B) Higher magnification panels of nuclei stained for DNA and CeCENP-C. The panels on the left show a prophase pronucleus just before pronuclear meeting. CeCENP-C localizes to a patchy stripe that runs along the partially condensed chromosomes. The panels on the right show a pronucleus after the pronuclei have met and the nuclear envelope has broken down. By this time, CeCENP-C localizes to two plates on opposite faces of the fully condensed chromosomes. All images are projections of three-dimensional stacks. Bars: (A) 5 um; (B) 1 um.
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CropCrop_pictureWBPicture0000008938
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>.
Publication_year2001
Article_URLDOIid10.1083/jcb.153.6.1209
Journal_URLTheJournalofCellBiology
Publisher_URLTheRockefellerUniversityPress
ReferenceWBPaper00004736