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WBPicture0000008878DescriptionFigure 4. Spatial and temporal expression pattern of mlt-10. (A) Representative fluorescence and Nomarski micrographs show expression of the mlt-10p::gfp-pest fusion gene. GFP was detected in the major body hypodermal syncytium (arrow), the dorsal and ventral ridges of the hypodermis (asterisk), and the anterior hypodermis (arrowhead) of the late L4 stage larva. All fluorescence images were acquired with an exposure time of 187 ms. (B) Detection of mlt-10 transcripts by RT-PCR. Larvae were cultured on bacteria that expressed the indicated dsRNAs for 40 hours at 25C. Animals were harvested at the typical time of the L4-to-adult molt. Detection of ama-1 transcripts controlled for the quality of RNA samples and RT-PCR reactions.
NameF4.large.jpg
DepictExpr_patternExpr9014
AnatomyWBbt:0005733
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2010
Article_URLDOIid10.1091/mbc.E08-07-0708
Journal_URLMolecularBiologyoftheCell
Publisher_URLTheAmericanSocietyForCellBiology
ReferenceWBPaper00036066