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WBPicture0000008674DescriptionFigure 8. Anti-UNC-13L antibody stains a form of UNC-13 protein that is synaptic but is unlikely to be on synaptic vesicles. C. elegans were double stained with anti-UNC-13L (left) and anti-UNC-17/VAChT (right). (A) Anti-UNC-13L in an adult wild-type head stains the major synaptic regions, including the nerve ring (NR), ventral nerve cord (VNC), dorsal nerve cord (DNC), and sublateral nerve cords (SNC). (B) Anti-UNC-17/VAChT stains a subset of the region stained by anti-UNC-13L. (C and D) A slightly higher magnification shows punctate staining with both antibodies in the dorsal nerve cord and sublateral nerve cords in the body. (E and F) A higher magnification shows the colocalization of anti-UNC-13L (E) and anti-UNC-17/VAChT (F) in synaptic regions (arrowheads) in one of the sublateral nerve cords in the head. (G and H) In an adult unc-104 mutant, staining of anti-UNC-13L is somewhat less intense but remains synaptic, whereas anti-UNC-17/VAChT is now localized in neuronal cell bodies (CB). Anterior is left; bars, 10 um.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2000
Article_URLDOIid10.1091/mbc.11.10.3441
Journal_URLMolecularBiologyoftheCell
Publisher_URLTheAmericanSocietyForCellBiology
ReferenceWBPaper00004372