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WBPicture0000008327 | Description | Figure 1. GFP expression pattern analysis of AQP-8.A, the aqp-8 1.6-kb upstream fragment drove GFP expression in the excretory cell (EC) and a secondary cell (L2). B, expression of a transgenic strain carrying the aqp-8p::GFP-PEST construct. Spatial expression patterns of the aqp-8p::GFP-PEST containing strains were effectively identical to the expression of strains carrying promoter::GFP constructs. C, expression of a stabilized transgene (genome-integrated tandem DNA array) leads to expression patterns that are identical to non-stabilized transgenic lines. D, closer analysis of an adult worm expressing the AQP::GFP construct (translational reporter fusion) shows that the GFP is localized to the outer walls of the excretory cell canal. The expression level of the translational construct was slightly lower than that of the transcriptional constructs (L3). E, expression of a stabilized transgene consisting of a 711-bp upstream fragment fused to GFP-PEST. All images were captured at 400x magnification. Camera conditions were the same for all images. Exposure times are as indicated on the GFP images (left images). | |||
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Name | F1.large.jpg | ||||
Depict | Expr_pattern | Expr4818 | |||
Anatomy | WBbt:0006850 | ||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2007 | ||||
Article_URL | DOI | id | 10.1074/jbc.M703305200 | ||
Journal_URL | TheJournalofBiologicalChemistry | ||||
Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
Reference | WBPaper00030881 |