WormBase Tree Display for Picture: WBPicture0000007978
expand all nodes | collapse all nodes | view schema
| WBPicture0000007978 | Description | Figure 6. Expression of nhr-23 reporter genes in epidermal cells during late embryogenesis and larval stages. (A) Schematic diagram of nuclear positions in a 1.5-fold embryo (adapted from Sulston, 1983) in which epidermal nuclei have been colored. Some of the epidermal nuclei form a distinctive set of three rows on either side of the embryo at this stage; the rows are composed of nuclei from Hyp7 (top row, yellow), V cells (middle row, blue) and P cells (bottom row, purple). Additional epidermal cells are located in the head and tail of the animal. (B,C) A lateral view of a comma-stage embryo harboring the transgene construct #4276 (see Fig. 4). The embryo was fixed and stained with the antibody MH27 (B) to visualize the borders of epidermal cells. GFP fluorescence (C) of the nhr-23 reporter marks the nuclei of all epidermal cells. (D-F) Comparison LIN-26 antibody staining of epidermal nuclei (D) to expression of a nhr-23::lacZ reporter gene (construct #3306) in a transgenic embryo (F) detected using an anti-β-galactosidase antibody. E is a merged image of LIN-26 and β-galactosidase-positive nuclei showing overlap of the two patterns in V and P nuclei (yellow). Not all epidermal nuclei are marked by the nhr-23 reporter gene due to mosaicism of expression. (G) A transgenic larva harboring a GFP-tagged nhr-23 gene (construct #4276) stained with MH27. Expression of the reporter gene in seam cells (epidermal) is indicated by small arrows; expression in tail epidermal cell is indicated by large arrows. Bar, ~10 µm. | |
|---|---|---|---|
| Name | FigL.jpg | ||
| Depict | Expr_pattern | Expr1429 | |
| Anatomy | WBbt:0005733 | ||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1998 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00003081 |
