WormBase Tree Display for Picture: WBPicture0000007890

WBPicture0000007890 Description: Fig. 4. drag-1 is expressed and functions in the same cell types as the Sma/Mab pathway components. (A-L)Images shown are side views with anterior to the left and posterior to the right. drag-1p::gfp (A-D) and sma-6p::rfp (E-H) colocalize in pharyngeal (A,E,I), hypodermal (B,F,J) and intestinal (C,G,K) cells, but do not colocalize in the male tail (D,H,L). I-L are merged images of A-D and E-H, respectively. (M-O)DRAG-1::GFP (M,N) or LIN-12TM::GFP (O; see Fig. 5 for details) localization in pharyngeal (M), hypodermal (N) and intestinal (O) cells, as visualized by anti-GFP antibody staining. Note that the GFP signal (green) is located outside of the nucleus (blue, stained with DAPI), both at the cell surface and inside the cell. Arrow heads, hypodermal cells; arrow, pharynx. (P)Tissue-specific rescue of the body size phenotype of drag-1(jj4) mutants. Body size was measured in adult worms at 72 hours post-plating. Transgenic worms were distinguished from non-transgenic ones by the presence of mec-7p::rfp. The lengths of drag-1(jj4) and non-transgenic worms of each group were normalized to 1. The relative lengths of wild-type and various transgenic animals compared with their corresponding non-transgenic controls are presented. Between 30 and 80 animals were measured for each genotype. Error bars represent 95% confidence intervals for the mean of relative body length. The significance of difference between transgenic and the corresponding non-transgenic animals was statistically analyzed via Student's t-test. ***, P<0.0001. (Q-U) The M lineage expression pattern of drag-1 using LIN-12TM::GFP (green; see Fig. 5 for details). Anti-FOZI-1 antibody staining (red) was used to mark M lineage cells from the 4-M to the 12-M stage (S-U). Only one focal plane was shown for the 8-M (T) and 12-M (U) stage worms. DRAG-1::GFP is present from the 1-M to the 4-M stage (O-S), then becomes fainter at the 8-M stage (T) and undetectable after the 8-M stage (U).
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  Crop: Crop_picture: WBPicture0000007891
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2010
    Article_URL: DOI id 10.1242/dev.051615
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00036372