WormBase Tree Display for Picture: WBPicture0000007792

WBPicture0000007792 Description: Figure 1. The DSL ligand lag-2 is expressed in the three pairs of IL2 neurons during the dauer stage. (A) A daf-7(e1372) dauer larva expressing the lag-2::GFP transgene and the corresponding DIC image overlaid with the GFP expression to show the position of the cells expressing the transgene. White arrowheads indicate the described expression of lag-2::GFP in the distal tip cells (DTC). Scale bar: 25 μm. (B) The head region of dauers expressing lag-2::GFP (qIs56) induced by either pheromone, or in various Daf-c mutants as indicated in the panels. (C) 3D reconstruction of a confocal stack of images of the IL2 neurons in dauer. White lines outline the pharynx of the dauer animal. Scale bars: 10 μm. (D) Diagram of the IL2 neurons indicating their position and their characteristic morphology (adapted with permission from wormatlas.org). (E) Merge of dauer expressing lag-2::GFP (green) in the IL2 neurons that were stained with the lipophilic dye DiI (red). As lag-2::GFP is expressed in the entire cell, whereas DiI only stains the membrane, colocalisation (yellow) is only observed at the membrane, giving a halo-like appearance. In all images, arrowheads indicate the IL2 neurons.
  Name: F1.large.jpg
  Crop: Crop_picture: WBPicture0000007793
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2008
    Article_URL: DOI id 10.1242/dev.012435
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00031997