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| WBPicture0000007678 | Description | Figure 4. lsy-2 is expressed ubiquitously and localizes to moving speckles in the nucleus. (A) lsy-2prom::gfp and lsy-2::gfp reporter gene constructs used in this study. The gray bar behind the green gfp sequence indicates the heterologous unc-54 3'UTR. (B) lsy-2prom::gfp and lsy-2::gfp reporter gene constructs are ubiquitously expressed at different developmental stages. Nine independent lsy-2::gfp transgenic lines and three independent lsy-2prom::gfp lines show similar expression patterns. (C) lsy-2::gfp is expressed in ASEL and ASER. ASEL and ASER are labeled with the ceh-36prom::rfp transgene otIs151. Note that the rfp reporter is diffusely localized throughout the cytoplasm, whereas the gfp signal is in nuclear speckles. lsy-2::gfp rescues the lsy-2 mutant phenotype (Table 3). (D) lsy-2::gfp is localized to moving nuclear speckles. Individual time frames of a movie, shot with Openlab Software at a time interval of half a second per frame (20 frames total), are displayed. The movie is available upon request. | |||
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| Name | F4.large.jpg | ||||
| Depict | Expr_pattern | Expr3799 | |||
| Anatomy | WBbt:0003903 | ||||
| WBbt:0003904 | |||||
| WBbt:0004017 | |||||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2005 | ||||
| Article_URL | DOI | id | 10.1242/dev.02163 | ||
| Journal_URL | Development | ||||
| Publisher_URL | TheCompanyofBiologists | ||||
| Reference | WBPaper00027031 |
