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| WBPicture0000007603 | Description | Figure 5. NAS-37::GFP protein is secreted. A C-terminal GFP fusion to NAS-37 expressed under the nas-37 promoter shows localization of NAS-37 protein. An overlay of a confocal projection of GFP fluorescence with the corresponding DIC image of an L4 worm (A) shows that NAS-37 is found in the cuticle and accumulates at the anterior end of the worm. It is also strongly expressed in the excretory duct cell (arrowhead). A confocal micrograph of the adult vulva (B) shows that NAS-37 accumulates in the vulval cuticle. A confocal projection of more lateral cuticle of an L4 animal (C) shows that NAS-37 is concentrated in the annular rings of the anterior cuticle, as well as in the lateral cuticle. A view of the posterior region of an L4 animal (D) shows that NAS-37 accumulates in the cuticle of the rectum. Fluorescence in the gut is due to autofluorescent particles and is not NAS-37::GFP. A confocal micrograph of a molted cuticle (E) shows that the NAS-37 protein accumulates in the anterior (arrowhead) and posterior (arrow) ends of the shed cuticle. | |||
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| Name | F5.large.jpg | ||||
| Depict | Expr_pattern | Expr3126 | |||
| Anatomy | WBbt:0004540 | ||||
| WBbt:0005755 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2004 | ||||
| Article_URL | DOI | id | 10.1242/dev.01454 | ||
| Journal_URL | Development | ||||
| Publisher_URL | TheCompanyofBiologists | ||||
| Reference | WBPaper00024579 |
