WormBase Tree Display for Interaction: WBInteraction000542944
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| WBInteraction000542944 | Interaction_type | Regulatory | Change_of_expression_level |
|---|---|---|---|
| Interactor | Interactor_overlapping_gene (13) | ||
| Interaction_summary | "We examined expression of 100 of the 104 up-regulated genes by qPCR and found 39 of 100 were reproducibly up-regulated twofold or greater after DNTBAR-1 overexpression in these experiments (data not shown). Of these, 13 were down-regulated 1.7-fold or more in response to expression of dominant negative DNTPOP-1 (class 1; Table 1), whereas nine were not (class 2; Table 1). The remaining BAR-1 responsive genes (17/39) were up-regulated upon both DNTBAR- and DNTPOP-1 overexpression, which is not expected for targets of the WBC or "canonical" Wnt pathway." | ||
| Detection_method | Construct | ||
| Transgene | |||
| RT_PCR | |||
| Regulation_level | Transcriptional | ||
| Paper | WBPaper00044857 | ||
| Remark | Table 1; "To conditionally activate Wnt signaling, we used hs::DNTbar-1, a transgene that uses the heat shock promoter to express a BAR-1 protein with an amino-terminal deletion (DNTBAR-1) predicted to stabilize the protein in the absence of Wnt signaling (Figure 1A) (Gleason et al. 2002); DNTpop-1 encodes a dominant negative variant of POP-1 in which the amino terminal beta-catenin binding domain is deleted (Korswagen et al. 2000)." |
