WormBase Tree Display for Interaction: WBInteraction000520124
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WBInteraction000520124 | Interaction_type | Regulatory | Change_of_expression_level |
---|---|---|---|
Interactor (2) | |||
Interaction_summary | The level of nhr-91 transcripts was increased in starved mir-235(n4504) L1 larvae, compared with wild type. In fed wild-type animals, significant upregulation of nhr-91 transcripts temporally coincides with downregulation of mature miR-235 after 8 and 10 h of feeding. Similarly, a reporter gene, in which the gfp-pest complimentary DNA was inserted between the promoter and the 3'UTR of nhr-91 (Pnhr-91::gfp- pest::nhr-91 3' UTR), also showed a burst of expression in the hypodermis, where Pmir-235::gfp was also expressed, after 8 to 10h of feeding. The level of nhr-91 transcripts was further increased in mir-235 mutants compared with wild-type animals after 8 h of feeding, suggesting that mir-235 functions not only to suppress expression of nhr-91 during L1 diapause, but also to limit the kinetics of its upregulation when feeding. | ||
Detection_method | Construct | ||
Reporter_gene | [nhr-91::gfp] | ||
Transgene | |||
RT_PCR | |||
Regulation_level | Post_transcriptional | ||
Paper | WBPaper00042363 |