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WormBase Tree Display for Expr_pattern: Expr9856

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Name Class

Expr9856Expression_ofGeneWBGene00007042
Reflects_endogenous_expression_ofWBGene00007042
Expression_dataLife_stageWBls:0000041
WBls:0000023
WBls:0000018
WBls:0000019
WBls:0000020
WBls:0000016
WBls:0000017
WBls:0000010
WBls:0000013
Anatomy_termWBbt:0008378Certain
WBbt:0008409Certain
GO_termGO:0005694
TypeReporter_gene[PBRM-1::gfp] translational fusion. The pbrm-1::gfp construct consisted of a genomic fragment from a cosmid C09B10 (a 2-kb fragment from 1.5-kb upstream of the start codon to the XbaI site) and a PCR fragment (XbaI site to the codon that corresponds to the C-terminus of PBRM-1). which was amplified from the yk1173e11 cDNA clone (a gift from Y. Kohara, National Institute of Genetics, Mishima, Japan) and ligated to the gfp gene from pPD95.75 (a gift from A. Fire).
PatternPBRM-1::GFP was expressed in the nuclei of most, if not all, somatic cells, including the T cell and its daughter cells. PBRM-1::GFP was first observed during the gastrulation stage and continues to be expressed throughout subsequent development and in the adult stage. During mitosis, PBRM-1::GFP was associated with chromosomes in the T cell and in other seam cells.
ReferenceWBPaper00040473
TransgeneWBTransgene00014750