WormBase Tree Display for Expr_pattern: Expr9856
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Expr9856 | Expression_of | Gene | WBGene00007042 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00007042 | |||
Expression_data | Life_stage | WBls:0000041 | ||
WBls:0000023 | ||||
WBls:0000018 | ||||
WBls:0000019 | ||||
WBls:0000020 | ||||
WBls:0000016 | ||||
WBls:0000017 | ||||
WBls:0000010 | ||||
WBls:0000013 | ||||
Anatomy_term | WBbt:0008378 | Certain | ||
WBbt:0008409 | Certain | |||
GO_term | GO:0005694 | |||
Type | Reporter_gene | [PBRM-1::gfp] translational fusion. The pbrm-1::gfp construct consisted of a genomic fragment from a cosmid C09B10 (a 2-kb fragment from 1.5-kb upstream of the start codon to the XbaI site) and a PCR fragment (XbaI site to the codon that corresponds to the C-terminus of PBRM-1). which was amplified from the yk1173e11 cDNA clone (a gift from Y. Kohara, National Institute of Genetics, Mishima, Japan) and ligated to the gfp gene from pPD95.75 (a gift from A. Fire). | ||
Pattern | PBRM-1::GFP was expressed in the nuclei of most, if not all, somatic cells, including the T cell and its daughter cells. PBRM-1::GFP was first observed during the gastrulation stage and continues to be expressed throughout subsequent development and in the adult stage. During mitosis, PBRM-1::GFP was associated with chromosomes in the T cell and in other seam cells. | |||
Reference | WBPaper00040473 | |||
Transgene | WBTransgene00014750 |