WormBase Tree Display for Expr_pattern: Expr9852
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| Expr9852 | Expression_of | Gene | WBGene00009738 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00009738 | |||
| Expression_data | Anatomy_term | WBbt:0004444 | Certain | |
| WBbt:0004445 | Certain | |||
| Type | Reporter_gene | [hecw-1] transcriptional fusion. The hecw-1 promoter region was amplified by using 5' primer 5'-ATCGTGGTTTCGCACTTTGTTTTGGAAAC-3' and 3' primer 5'-TCTCCGAAGCGTGATCATTCTAAAAACC-3'. The 900-bp fragment was subsequently cloned into the pPD95.75 vector (Fire Lab Vector Kit, Addgene) to generate the hecw-1 promoter GFP reporter. --precise ends. | ||
| Pattern | GFP fluorescence was observed in the nervous system throughout the body, but in the anterior ganglion was conspicuously limited to two neurons located posterior to the anterior bulb of the pharynx, each of which have projections extending anteriorly. We confirmed that these two neurons are the lateral outer labial neurons OLLL and OLLR by co-localization experiments using the OLL-specific reporter ser-2dp::gfp21 and the hecw-1p::mCherry transgene. | |||
| Reference | WBPaper00040442 | |||
| Transgene | WBTransgene00014748 |
