WormBase Tree Display for Expr_pattern: Expr9237
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| Expr9237 | Expression_of | Gene | WBGene00019946 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00019946 | |||
| Expression_data | Anatomy_term | WBbt:0005772 | Certain | |
| GO_term | GO:0005886 | |||
| GO:0055037 | ||||
| GO:0031901 | ||||
| GO:0055038 | ||||
| Subcellular_localization | The subcellular localization of CHAT-1 in the intestine was determined by expressing CHAT-1::CFP under control of the intestine-specific promoter vha-6 (Pvha-6chat-1::cfp). CHAT-1 was localized to both plasma membranes and intracellular tubular and vesicular structures and colocalized with TAT-1. The intestinal tubular and vesicular localization pattern was also observed when the expression of CHAT-1 was controlled by the endogenous promoter. | |||
| To determine the identities of the cytosolic compartments labeled by CHAT-1, mCHERRY fusions of different endocytic markers were coexpressed together with CHAT-1::GFP and TAT-1 (Pvha-6chat-1::gfp +Pvha-6tat-1). TAT-1 was included to ensure efficient ER export of CHAT-1::GFP; this combination is subsequently referred to as CHAT-1::GFP for simplicity. CHAT- 1::GFP displayed a tubular and vesicular staining pattern, which did not overlap with either the Golgi marker MANS or the lysosomal marker Lysotracker Red, indicating that CHAT-1 is not on the Golgi or mature lysosomes. No CHAT-1::GFP was found on the RAB-7-positive ring-like structures, suggesting that it is not enriched on late endosomes or early lysosomes. CHAT-1 partially overlapped with RAB-5 on punctate structures, but not on tubule-like structures that were negative for mCHERRY::RAB-5. Thus, a proportion of CHAT-1/ TAT-1 may localize to RAB-5-positive early endosomes. CHAT-1/TAT-1 associates with the tubular membrane of early endosomes and ERCs. CHAT-1::GFP overlapped with mRFP::RME-1 on basolateral tubulo-vesicular structures, indicating that CHAT-1 also localizes to RME-1- positive recycling endosomes. | ||||
| Type | Reporter_gene | |||
| Picture | WBPicture0000012421 | |||
| WBPicture0000012422 | ||||
| Reference | WBPaper00037899 | |||
| Transgene | WBTransgene00031023 |
