WormBase Tree Display for Expr_pattern: Expr8718
expand all nodes | collapse all nodes | view schema
| Expr8718 | Expression_of | Gene | WBGene00016961 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00016961 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005175 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005733 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0006748 | Certain | |||
| GO_term | GO:0010008 | |||
| Subcellular_localization | In embryo, CeVPS-32 immunoreactivity was detected in a diffuse cytosolic pattern with a punctiform component characteristic of vesicular localisation. To confirm that CeVPS-32 positive vesicles are endosomes authors looked for colocalization with the endosomal marker CeVPS-27/HGRS-1. Vesicles positive for both CeVPS-32 and CeVPS-27 were detected in all epithelial tissues. However, only a subset (30%, n=606) of vesicles is positive for both markers suggesting that the presence of CeVPS-27 and/or CeVPS-32 correspond to distinct stages of endosomal maturation. | |||
| Type | Antibody | |||
| Pattern | During larval development CeVPS-32-positive vesicles are still detected in epithelial tissues, such as the epidermis, the pharynx, the intestine, the vulva, the somatic gonad and the spermatheca. In addition, there was an accumulation of punctuate CeVPS-32 expression in the male germ line in both hermaphrodites and male adults. A careful analysis revealed that CeVPS-32 is not localized in germ cells but in residual bodies which are transient cytoplasts formed between the second meiotic division of secondary spermatocytes and formation of spermatids. | |||
| During the rapid cell divisions which characterize the first half of embryogenesis, the number of CeVPS-32 positive vesicles strongly increases and the size of the vesicles appears smaller. During the second half of embryogenesis, when cells stop dividing and organogenesis proceeds, the CeVPS-32 vesicles are enriched in epithelial tissues such as the intestine, the pharynx and the epidermis. The pattern in pharynx and intestine coincided with the appearance of apical vesicles observed by EM and suggests that they are involved in lumen formation. | ||||
| Immunohistochemical analyses with the anti-CeVPS-32 antibodies revealed that the protein is ubiquitously expressed and detected as early as the 4-cell stage during embryogenesis. Expression is maintained throughout embryonic development, and during larval and adult stages. | ||||
| Picture | WBPicture0000011968 | |||
| WBPicture0000011969 | ||||
| WBPicture0000011970 | ||||
| Remark | Picture: Fig 2, Fig 3. | |||
| The specificity of anti-CeVPS-32 antibodies was confirmed by the almost complete extinction of the signal after inactivation of Cevps-32 by RNA interference and in the Cevps-32(ok1355) null mutant. | ||||
| Reference | WBPaper00033139 | |||
| Antibody_info | WBAntibody00001923 |
