WormBase Tree Display for Expr_pattern: Expr8600
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| Expr8600 | Expression_of | Gene | WBGene00004793 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004793 | |||
| Expression_data | Anatomy_term | WBbt:0004103 | Certain | |
| WBbt:0004104 | Certain | |||
| GO_term | GO:0030425 | |||
| GO:0009986 | ||||
| Subcellular_localization | In PLM neurons obtained from Pmps-1::wild type mps-1::gfp embryos, Both MPS-1 and KHT-1 were detected in the dendrite and along the perimeter of the cell, indicating surface expression as expected. Most importantly, their signals overlapped. | |||
| Type | Antibody | Transgenic worms expressing the wild-type mps-1 gene and a mutated gene encoding for the inactivated kinase fused to gfp in the C-terminus in a mps-1 KO background (Pmps-1::wild type mps-1::gfp and Pmps-1::d178n mps-1::gfp) were stained with anti-KHT-1 antibody. Both constructs gave strong GFP signals in the cells where MPS-1 is normally expressed, including the PLM neurons (Supplementary Figure S-1A-B), indicating that wild-type and D178N fold and traffic to the plasma membrane normally and that inactivation of the kinase activity of MPS-1 has no effect on its expression. | ||
| Remark | Picture: Figure 3B, 3C, 3D, Supplementary Figure S-2. | |||
| Reference | WBPaper00033073 | |||
| Antibody_info | WBAntibody00001905 |
