WormBase Tree Display for Expr_pattern: Expr8581
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| Expr8581 | Expression_of | Gene | WBGene00010444 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00010444 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term (16) | |||
| GO_term | GO:0043005 | ||
| Subcellular_localization | Anti-MAGI-1 antibody detected MAGI-1 localization to puncta along the ventral cord; however, MAGI-1 is expressed in more neurons than just those that express GLR-1, making analysis of colocalization in the ventral cord difficult. | ||
| To help restrict the analysis to the GLR-1-expressing neurons, both MAGI-1 isoforms were expressed as mRFP chimeric proteins under the glr-1 promoter; both substantially colocalized with GLR-1. | |||
| Type | Antibody | ||
| Reporter_gene | |||
| Pattern | The expression of genomic magi-1::gfp and magi-1::rfp chimeric transgenes, which contain 4 kb of upstream sequence and either the complete magi-1 ORF or only the genomic magi-1 long isoform sequences, yielded similar results | ||
| The magi-1L:::gfp reporter transgene reflects the expression of the long isoform. Expression of MAGI-1L::GFP was detected in the pharynx, head neurons, ventral cord, and vulva of animals mosaic for the extrachromosomal array. Expression of MAGI-1L::RFP was also detected in the pharynx, intestine, vulval epithelia (L4 stage), and vulval muscle (adult stage) of animals mosaic for the extrachromosomal array. | |||
| This antibody labeled both neurons and epithelial junctions in immunofluorescence experiments. Within the hypodermis and intestine, there was a high degree of colocalization between MAGI-1 and DLG-1. The anus and vulva also were labeled in larval and adult animals. Finally, MAGI-1 expression was observed in some neurons in the head, including a subset of the GLR-1::GFP-expressing command interneurons. MAGI-1 is expressed in the backward but not the forward command interneurons; MAGI-1 expression was not detected in any of the mechanosensory neurons. MAGI-1 was detected in the neurons AVA, AVD, and SMD, as well as RMD and RMDV. | |||
| Picture | WBPicture0000007366 | ||
| WBPicture0000007368 | |||
| Remark | Picture: Figure 2, Fig. S2BE. | ||
| Reference | WBPaper00032939 | ||
| Transgene | WBTransgene00030574 | ||
| WBTransgene00031678 | |||
| WBTransgene00031679 | |||
| Antibody_info | WBAntibody00001733 |
