WormBase Tree Display for Expr_pattern: Expr8115
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Expr8115 | Expression_of | Gene | WBGene00003830 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003830 | |||
Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
WBbt:0005319 | Certain | |||
WBbt:0005733 | Certain | |||
WBbt:0005745 | Certain | |||
WBbt:0005751 | Certain | |||
WBbt:0005772 | Certain | |||
WBbt:0006749 | Certain | |||
WBbt:0006760 | Certain | |||
GO_term | GO:0005886 | |||
GO:0005737 | ||||
Subcellular_localization | At the subcellular level, in most cells the protein accumulated in punctate structures within the cytoplasm. Some protein was present very close to the plasma membrane and may be associated with it. In polarized epithelia, expression of the protein was predominantly on the baso-lateral parts of the cell and was largely absent from the apical cell membrane or the apical cytoplasm. In the intestine, seam cells, ventral hypodermis, pharynx, and other polarized epithelial cells, the most apical boundary of expression coincided with the adherens junctions that separate baso-lateral and apical cell membranes. Localization of the GFP fusion protein close to the junctions in the intestine and seam cells was observed. | |||
Type | Reporter_gene | |||
Pattern | The expression pattern seen was the same in all eight lines. Strong expression of GFP was first detected in the embryo at the E16 stage in the intestine primordium. In later stage embryos, expression was also seen in other epithelial tissues. In larval worms, and throughout the adult stage, expression was seen in the intestine, pharynx, hypodermis, seam cells, spermatheca, and uterus. During postembryonic development, several nonepithelial cells also expressed the transgene, including many neurons in the nerve ring and coelomocytes, scavenger cells situated in the pseudocoelom. Within the nervous system, expression was not uniform but higher in a subset of neurons. | |||
Picture | WBPicture0000010990 | |||
Remark | Picture: Figure 1, and S1. | |||
Reference | WBPaper00031894 | |||
Transgene | WBTransgene00004640 | |||
WBTransgene00004641 | ||||
WBTransgene00004642 | ||||
WBTransgene00004643 | ||||
WBTransgene00004644 |