WormBase Tree Display for Expr_pattern: Expr544
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| Expr544 | Expression_of | Gene | WBGene00003001 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003001 | ||
| Expression_data | Life_stage | WBls:0000035 | |
| Anatomy_term (17) | |||
| GO_term | GO:0005886 | ||
| GO:0016324 | |||
| Subcellular_localization | In Z1.ppp and Z4.aaa, and in other gonadal cells, LIN-12::GFP is detectable over the entire surface of cells. However, in the VPCs, LIN-12::GFP is visible in the perinuclear region, which is probably endoplasmic reticulum/Golgi, and at the ventral (apical) surface and at the junctions between VPCs. | ||
| Type | Reporter_gene | ||
| Pattern | LIN-12::GFP protein is initially detectable in both Z1.ppp and Z4.aaa, and later in development becomes detectable in the presumptive VU but not in the presumptive AC. The LIN-12:: GFP protein is initially detectable in all six VPCs. However, in the mid-L3 stage, at the time of VPC specification, LIN-12::GFP is reduced in P6.p relative to the other VPCs, notably with respect to P5.p and P7.p. High LIN-12::GFP accumulation is always seen in the daughters of P5.p and P7.p, but LIN-12::GFP accumulation is not seen in the daughters of P6.p. There is variable accumulation of LIN-12::GFP in the daughters of P3.p, P4.p and P8.p. | ||
| Picture | WBPicture0000007992 | ||
| WBPicture0000007993 | |||
| Remark | Starting plasmid pLB30 fully rescues lin-12(null) mutant. Integrated strain arIs41[lin-12::GFP] yields mostly wild-type hermaphrodites, with <5% showing weak lin-12 gain-of-function phenotype. | ||
| Reference | WBPaper00003203 | ||
| Transgene | WBTransgene00000048 |
