WormBase Tree Display for Expr_pattern: Expr4872
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Expr4872 | Expression_of (2) | |||
---|---|---|---|---|
Expression_data | Anatomy_term | WBbt:0004506 | Certain | |
WBbt:0004520 | Certain | |||
WBbt:0005813 | Certain | |||
WBbt:0006748 | Certain | |||
Type | Reporter_gene | |||
Pattern | In each line, the pat-2p::GFP nematodes had high levels of GFP expression in body-wall muscle cells and vulval tissue. No GFP was detected in the DTCs migrating on the ventral ECM during L2. GFP was visible in DTCs during L3, when the DTC migrates from the ventral to dorsal surface, and was maintained in the DTC throughout adulthood. | |||
Picture | WBPicture0000008516 | |||
Remark | Picture: Figure 2. | |||
Similar DTC expression of GFP was observed in two other independently derived 6-kb pat-2 promoter fusion lines as well as with shorter promoter fusions of 3, 2, and <0.5 kb of pat-2 upstream sequence. To verify that this pattern is an accurate representation of PAT-2 expression, a translational fusion line with GFP inserted near the end of the cytoplasmic tail was examined. The PAT-2::GFP expression pattern was the same as the transcriptional fusions, including the up-regulation of expression in the DTC during L3. | ||||
Reference | WBPaper00030828 | |||
Transgene | WBTransgene00029511 | |||
Associated_feature | WBsf027927 |