WormBase Tree Display for Expr_pattern: Expr3754
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| Expr3754 | Expression_of | Gene | WBGene00004393 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004393 | |||
| Homol | Homol_homol | B0414:Expr | ||
| Expression_data | Life_stage (11) | |||
| Anatomy_term | WBbt:0005753 | Certain | ||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | Expressed in the nuclei | |||
| Type | Reporter_gene | |||
| Pattern | At the pre-comma stage, at about 260 min of embryonic development, RNT-1::GFP expression began in the nuclei of the seam cells (H0-2, V1-6 and T cells). At the comma stage, at about 400 min, expression also appeared in the body wall muscle cells . At the L1 larval stage, RNT-1::GFP expression was observed in the seam cells, the body wall muscle cells, and occasionally in the intestinal cells. In the H, V, and T cell lineages, RNT-1::GFP expression was always observed in the seam (self-renewing) cells, but not in the hypodermal syncytial (differentiating) cells, from L1 to adult stages. In addition, RNT-1::GFP expression was observed in the descendants of the M blast cell from late L1 to adult stages. The expression began after two rounds of M cell division, and continued in the body wall muscle descendants. | |||
| RNT-1::GFP was strongly expressed in the T cells until just before the initiation of mitosis. The expression was diminished in prophase and not visible from metaphase to telophase of the T cell division. The expression was not detectable in either daughter of the T cells, T.a and T.p, but reappeared in the posterior daughters of the T.a cells, T.ap, several hours after the T.a cell division in late L1 stage. After this stage, RNT-1::GFP expression was exclusively observed in T.app and T.appa, but not in either of the other T.ap descendants or any T.p descendants. | ||||
| Remark | RNT-1::GFP expression was not observed in T.a and T.p by anti-GFP antibody staining to enhance the GFP signal intensity, or by cultivating worms at 15 centigrades for accumulation and maturation of GFP in T.a and T.p. | |||
| Reference | WBPaper00026860 | |||
| Transgene | WBTransgene00001017 | |||
| WBTransgene00001018 |
