WormBase Tree Display for Expr_pattern: Expr3651
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| Expr3651 | Expression_of | Gene | WBGene00003377 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003377 | ||
| Expression_data | Anatomy_term (16) | ||
| GO_term | GO:0005634 | ||
| Subcellular_localization | Localized in the nucleus. | ||
| Type | Antibody | ||
| Reporter_gene | |||
| Pattern | The GFP::MLS-2 fusion construct and antibody staining showed identical expression patterns. Expression of MLS-2 was first detectable in one or two cells in embryos at the 50-cell stage and is localized in the nucleus. MLS-2 continued to be expressed in proliferating cells that are primarily located at the anterior of the embryo and are presumably derived from the AB lineage. During morphogenesis, this expression became restricted to a small subset of head neuronal precursors. Expression persisted in six head neurons during postembryonic development. GFP::MLS-2 expression was also observed in unidentified cells near the vulva at the L2 and L3 stages. | ||
| To characterize the M lineage expression pattern of mls-2, double-labeling experiments were performed using anti-MLS-2 antibodies and the M lineage-specific hlh-8::gfp or hlh-8::lacZ markers. mls-2 expression in the M lineage was first detectable in the M mesoblast, and was retained during the first three rounds of cell divisions, such that mls-2 expression was still detectable in eight M descendants (designated 8-M stage, n>200). However, after one more round of cell division (at the 16-M stage), no MLS-2 signal was detected either by anti-MLS-2 antibodies or by the gfp::mls-2 fusion construct. Although it is possible that a low level of MLS-2 protein is present after the 8-M stage, the loss of MLS-2 signal at the 16-M stage appears to be due to the instability of the MLS-2 protein, because the mls-2 promoter is still active in M lineage descendants after the fourth round of cell division, as detected by a transcriptional mls-2p::gfp::mls-2 3' UTR construct. Neither the mls-2 promoter activity nor the MLS-2 protein was detected in the SM lineage or the differentiated BWMs and CCs. Thus mls-2 is expressed in the proliferating cells of the early M lineage. | |||
| Picture | WBPicture0000007653 | ||
| Reference | WBPaper00026712 | ||
| Transgene | WBTransgene00028790 | ||
| Antibody_info | WBAntibody00000863 |
