WormBase Tree Display for Expr_pattern: Expr3212

Expr3212 Expression_of: Gene: WBGene00004020
    Reflects_endogenous_expression_of: WBGene00004020
  Expression_data: Anatomy_term: WBbt:0005772 Partial
  Type: Reporter_gene
  Pattern: Expression of the transgenic reporter construct is gut-specific, can first be detected late in embryogenesis and shows anterior - posterior patterning that is, to a reasonable approximation, the same as that detected by the histochemical assay for endogenous PHO-1 activity. In ~70% of newly hatched larvae, reporter expression is undetectable in the six cells belonging to int1 and int2 but strong in more posterior cells of the intestine. In the remaining ~30% of transgenic larvae, low levels of reporter expression can be detected in the four int1 cells; reporter expression is only rarely detected in the two int2 cells or in any cells outside of the intestine. Expression in the most posterior pair of intestinal cells (int9) appears to be weaker and more variable than in anterior neighbors.
  Remark: Given the technical limitations of the histochemical assay for acid phosphatase activity (e.g., incomplete permeabilization and potential diffusion of the reaction product), it is difficult to rule out a similar low level of endogenous PHO-1 activity in a fraction of int1 cells or to claim with certainty that endogenous PHO-1 activity does indeed extend completely to the gut posterior.
  Reference: WBPaper00024976
  Transgene: WBTransgene00000345