WormBase Tree Display for Expr_pattern: Expr3127
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| Expr3127 | Expression_of | Gene | WBGene00004963 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004963 | |||
| Expression_data | Anatomy_term | WBbt:0006800 | Certain | |
| GO_term | GO:0005737 | |||
| GO:0031143 | ||||
| Subcellular_localization | SPE-9 is concentrated in spikes during spermiogenesis and pseudopods in mature sperm. | |||
| SPE-9 is segregated to spermatids where, depending on the plane of focus, a ring of staining was seen. In addition to the identical staining patterns observed with the EX and C antisera, sperm isolated from worms carrying a null allele of spe-9 was stained to further validate specificity. spe-9(eb19) animals produce spermatids that show no immunoreactivity to both the EX and C sera. The SPE-9 staining pattern is very different from staining patterns seen with sera that stain internal sperm structures. Spermatids were also labeled with the monoclonal antibody 1CB4. The epitope recognized by 1CB4 has been shown to reside on membranous organelles (MOs). MOs are located in the cytoplasm close to the plasma membrane. This MO vesicle-staining pattern is distinct from SPE-9 staining. | ||||
| Type | Antibody | |||
| Picture | WBPicture0000012019 | |||
| WBPicture0000012020 | ||||
| WBPicture0000012021 | ||||
| Reference | WBPaper00024323 | |||
| Antibody_info | WBAntibody00000886 | |||
| WBAntibody00000887 |
