WormBase Tree Display for Expr_pattern: Expr3094

Expr3094 Expression_of: Gene: WBGene00006775
    Reflects_endogenous_expression_of: WBGene00006775
  Expression_data: Life_stage: WBls:0000024
      WBls:0000003
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term: WBbt:0004489
      WBbt:0004574
      WBbt:0004577
      WBbt:0004947
      WBbt:0004949
      WBbt:0005394 Certain
      WBbt:0005751 Partial
        Remark: Expressed transiently in 4 embryonic coelomocytes
      WBbt:0005813 Certain
      WBbt:0008594 Certain
  Type: Reporter_gene
  Pattern: At approximately 240 min post-fertilization, UNC-39::GFP appeared in more posteriorly located nuclei in addition to the anterior nuclei. Posterior nuclei expressing unc-39::gfp included Z1 and Z4, the M cell, and the embryonic coelomocytes. Occasionally expressed in additional posteriorly located cells. Expression in M and the coelomocytes was transient and no longer observed by 600 min post-fertilization, whereas Z1 and Z4 expression persisted into the L1 larval stage.
    Expression of the full-length unc-39::gfp transgene was dynamic throughout development. UNC-39::GFP was first seen at approximately 100 min post-fertilization when gastrulation and embryonic transcription begin. UNC-39::GFP accumulated in the nuclei of 12 to 16 blast cells in the anterior of the embryo. As embryogenesis proceeded, expression was observed in increasing numbers of anterior nuclei which were most likely the descendants of the earlier-expressing cells, as UNC-39::GFP-expressing pairs of cells in the process of cell division were observed. Expression in anterior nuclei continued until about 450 min post-fertilization (2-fold stage), at which time expression became restricted to less than 10 cells and persisted into adulthood. The locations and morphologies of some of these cells indicated that they were anterior neuroblasts derived from the AB lineage.
    In addition to expression in the anteriorly generated mesodermal Z1, Z4, M, and coelomocytes, the unc-39 promoter was active in all of the body wall muscle cells of late embryos and L1 larvae. Thus, unc-39::gfp transgenes were expressed very early in embryogenesis in anterior cells that generate neuronal and mesodermal tissues, and later in body wall muscle cells.
    The 4-kb unc-39 promoter driving the expression of gfp alone showed a similar general pattern. However, expression of this construct persisted in a greater number of anterior cells into larval and adult stages. These cells had axon and dendrite morphologies characteristic of amphid sensory neurons. Furthermore, the CAN neuron clearly showed unc-39 promoter expression.
  Reference: WBPaper00024327
  Transgene: WBTransgene00028352