WormBase Tree Display for Expr_pattern: Expr3085
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| Expr3085 | Expression_of | Gene | WBGene00006604 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006604 | |||
| Expression_data | Anatomy_term | WBbt:0004574 | ||
| WBbt:0004577 | ||||
| WBbt:0005792 | Certain | |||
| WBbt:0007854 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| Subcellular_localization | Expressed in nuclear and cytoplasm. | |||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | After hatching, TRA-1 was still detected in SGPs, but its level and subcellular distribution were distinct in the two sexes. In hermaphrodite L1 SGPs, TRA-1 was largely nuclear, but in male L1 SGPs, TRA-1 was present at a lower level and was uniformly distributed between nucleus and cytoplasm. A similar sexually dimorphic difference was observed in the distribution of GFP::TRA-1 in L1 SGPs. The GFP::TRA-1 reporter partially rescued tra-1(0) mutants, indicating that it produces a functional protein. After the first SGP division, TRA-1 continues to be expressed in hermaphrodite gonads, but is no longer detectable in males. | |||
| In wild-type XX embryos, TRA-1 was predominantly nuclear in SGPs from formation of the primordium, through embryogenesis, and into the first larval stage. | ||||
| Next, embryos from a fog-2 male/female strain was examined , which produces 50% XX and 50% XO progeny, to determine if TRA-1 is similarly expressed in male SGPs. In 2-fold embryos, most SGPs possessed nuclear TRA-1 (27/30). Therefore, TRA-1 is present in SGP nuclei of both XX hermaphrodite and XO male embryos. | ||||
| Picture | WBPicture0000007594 | |||
| Reference | WBPaper00024274 | |||
| Transgene | WBTransgene00028345 | |||
| Antibody_info | WBAntibody00000775 | |||
| WBAntibody00000776 |
