WormBase Tree Display for Expr_pattern: Expr2974

Expr2974 Expression_of: Gene: WBGene00006546
    Reflects_endogenous_expression_of: WBGene00006546
  Expression_data: Life_stage: WBls:0000005
      WBls:0000010
    Anatomy_term (12)
  Type: In_situ
  Pattern: A hybridization signal was first detected in the nucleus of the E cell at the 8-cell stage, indicating the onset of zygotic expression of tbx-9 in this cell. Expression in the E cell was retained in its daughter cells Ea and Ep until the 24-cell stage. It then became undetectable. At this stage, signals were detected in five more cells.
    After this stage, signals were observed in a subset of cells at each stage up to the approximately 400-cell stage and became undetectable just before the onset of morphogenesis. The most intensive hybridization signals were found in four cells at the approximately 200-cell stage. These cells were arranged as two pairs of two cells near the center of embryos, a location usually occupied by MS descendant cells.
    For exact orientation, embryos were subjected to double staining by fluorescence in situ hybridization for the tbx-9 mRNA along with marker mRNAs. Two of the tbx-9 expressing cells were identified as the Ca and Cp cells. Double staining by fluorescence in situ hybridization for tbx-9 and elt-1 revealed the remaining three tbx-9 expressing cells were AB descendant cells destined for hypodermal cells.
  Reference: WBPaper00006477