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WormBase Tree Display for Expr_pattern: Expr2686

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Name Class

Expr2686Expression_ofGeneWBGene00000915
Reflects_endogenous_expression_ofWBGene00000915
Expression_dataLife_stageWBls:0000003
WBls:0000041
WBls:0000057
Anatomy_termWBbt:0004575Certain
WBbt:0004576Certain
WBbt:0005739Certain
WBbt:0005784Certain
WBbt:0007850Certain
TypeIn_situIn situ hybridization with sense and antisense daf-21 DIG-labeled RNA probes
PatternIn adult males, daf-21 mRNA was detected in germline cells, similarly as in hermaphrodites, with a strong signal seen in spermatogonia, decreasing signal in spermatocytes, and none in mature sperm.
In the embryo comma stage, daf-21 mRNA was strongly detected in cells of the head region and less so in other areas. In the hatched L1 larvae, daf-21 mRNA was mainly distributed in the germline precursor cells Z2 and Z3 and the head region, whereas in the adult hermaphrodite, it was localized uniquely in the germ cells. In the distal arms of the adult hermaphrodite, mitotically dividing germline cells were strongly stained with the antisense probe, but not with the sense probe. As the oocytes matured, the signal strength of the antisense probe seemed to decrease.
ReferenceWBPaper00006057