WormBase Tree Display for Expr_pattern: Expr2601
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| Expr2601 | Expression_of | Gene | WBGene00006876 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006876 | |||
| Expression_data | Anatomy_term | WBbt:0005175 | Partial | |
| WBbt:0005733 | Partial | |||
| WBbt:0005790 | Certain | |||
| WBbt:0005791 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0006749 | Certain | |||
| GO_term | GO:0005882 | |||
| Subcellular_localization | The circumferential bands formed by VAB-10A matched those formed by IFs at all stages. VAB-10A staining strictly colocalized with myotactin in adults, but only partially in young larvae. The parallel bands formed by VAB-10B are interspersed with those formed by VAB-10A and extend beyond the area of muscle epidermis contact. The VAB-10B bands corresponded to the regularly spaced shallow furrows that pattern the cuticle and separate annuli ridges. Immunoelectron microscopy confirmed that VAB-10A (but not VAB-10B) is restricted to FOs. VAB-10A was exclusively detected in the epidermis in areas dense in filaments, a characteristic of FOs. Using the VAB-10B K22 antibodies, gold particles were observed in the epidermis adjacent to body wall muscles and in sarcomeres. Although the number of particles is low, quantitative analysis indicates that staining is specific. Thus, VAB-10B appears to have a disperse distribution in the epidermis and muscles. | |||
| Type | Antibody | VAB-10 rabbit polyclonal antibodies. To produce antibodies, GST fusions with RT-PCR fragments encoding VAB-10A residues G2837-Q3436, VAB-10B residues D2697-R3862, or VAB-10B residues E3863-K4955 were purified using DEAE-Sepharose followed by glutathione-Sepharose 4B columns, and were used to immunize two male New Zealand rabbits. | ||
| Pattern | In embryos, VAB-10A and VAB-10B antibodies first detected a signal at the basal and apical plasma membranes of dorsal and ventral epidermal cells, soon after the onset of differentiation. As these cells became thinner during morphogenesis, staining appeared as four longitudinal rows and progressively evolved into circumferentially oriented bands located above muscle sarcomeres. VAB-10A was also detected basally and apically in the pharynx and along mechanosensory axons. VAB-10B was also present in the pharynx lumen, intestine lumen, nerve ring, and in body wall muscles and somatic gonad of larvae. | |||
| Picture | WBPicture0000008960 | |||
| WBPicture0000008961 | ||||
| Remark | These patterns are specific because MH5 and polyclonal VAB-10A antibodies failed to detect a signal in vab-10(h1356) and vab-10Adeficient embryos. Likewise, VAB-10B antibodies could only detect a weak signal in the intestine and pharynx of mid-staged vab-10B(mc44) and vab-10(h1356) embryos. Although it cannot be excluded that VAB-10B antisera recognize a cross-reacting protein, VAB-10B is likely to be expressed in the pharynx and the intestine because surviving vab-10B(mc44) larvae had an abnormal intestine. | |||
| Reference | WBPaper00005913 | |||
| Antibody_info | WBAntibody00000627 |
