WormBase Tree Display for Expr_pattern: Expr2347
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| Expr2347 | Expression_of | Gene | WBGene00000871 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000871 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term (41) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | nuclear | ||
| Type | Antibody | Mouse monoclonal antibodies that recognize CYE-1. Immunofluorescence was performed on fixed specimens. | |
| Pattern | CYE-1 is present in adult animals and is restricted to the germline, which is the only proliferative tissue in adults. CYE-1 levels vary in the germline. Mitotic germ cells in the distal region of the gonad have easily detectable levels of nuclear CYE-1. Germ cells in the initial stages of meiosis (proximal to the mitotic germ cells) have lower CYE-1 levels. Finally, as oocytes cellularize in the loop region of the gonad, CYE-1 levels increase with mature oocytes having the highest levels of nuclear CYE-1. These results demonstrate that a significant portion of maternal cye-1 contribution to the embryo is CYE-1 protein. | ||
| CYE-1 level was assayed postembryonically to determine whether CYE-1 could be detected and if levels of CYE-1 correlated with mitotic proliferation. CYE-1 protein is detectable in larval blast cells that give rise to all tissue types, including, germline, intestine, hypodermis, neurons, and muscle. During larval stages, the level of CYE-1 protein is much lower than that found in germ cells or in the early embryo. CYE-1 antibody staining is restricted to the developmental time when the blast cells are undergoing active proliferation. For example, in the L1 stage, proliferating P blast cells that produce ventral nerve cells have relatively high levels of nuclear CYE-1. In contrast, during the L2 larval stage, the nonproliferating neuronal descendents of the P blast cells have CYE-1 levels that are only barely detectable above background. Further, while a subset of the P cell descendents, the vulva precursor cells (VPCs), will proliferate in the L3 larval stage to produce the vulva, these cells do not have appreciable CYE-1 levels while they are quiescent in the L2 larval stage. Nuclear CYE-1 becomes detectable in the VPCs during the L3 larval stage when they begin proliferation. CYE-1 becomes undetectable in the VPC descendents after completion of cell divisions in L4 larval stage animals. | |||
| Monoclonal anti-CYE-1 antibody was used to assay CYE-1 levels from fertilization to the end of embryogenesis. In the zygote, CYE-1 is observed in the maternal and paternal pronuclei as soon as they form. The specificity of antibody staining was confirmed by cye-1 RNAi treatment of adult hermaphrodites that abolishes both oocyte nuclei and embryonic anti-CYE-1 protein staining. In early embryos, CYE-1 is enriched in nuclei, and levels appear constant with no evidence of cell cycle fluctuations other than during mitosis. During mitosis, CYE-1 antibody staining appears diffuse once nuclear envelope breakdown occurs, but resumes nuclear localization upon reformation of the nuclear envelope in telophase. CYE-1 is present equally in all cells of the early embryo. The level of CYE-1 declines during embryogenesis and disappears from most cells in comma-stage embryos coincident with the completion of the majority of embryonic cell divisions. | |||
| Reference | WBPaper00005708 | ||
| Antibody_info | WBAntibody00000602 |
