WormBase Tree Display for Expr_pattern: Expr2277
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Expr2277 | Expression_of | Gene | WBGene00001186 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00001186 | ||
Homol | Homol_homol | F55A8:Expr | |
Expression_data | Life_stage | WBls:0000038 | |
WBls:0000027 | |||
WBls:0000035 | |||
Anatomy_term (38) | |||
Type | Reporter_gene | ||
Pattern | The reporter construct (pKK63) is sufficient to drive GFP expression in the VPCs and their descendants as well as in the six VC motoneurons that innervate vulval muscles, which are likely to be co-regulated with vulval cells. When expression of egl-18::GFP is first detected in VPCs around the mid-L2 stage, all six VPCs are equally likely to express GFP. However, beginning at around the late-L2/early-L3 stage, until the VPCs divide in the mid-L3 stage, the expression in P5.p-P7.p is generally higher than in the other VPCs, and P6.p often shows the strongest expression. Expression persists in the descendants of P5.p-P7.p through the L4 stage, and P6.p descendants typically show stronger expression than descendants of P5.p and P7.p. This pattern is similar to that of lin-39 expression in the developing vulva. | ||
Picture | WBPicture0000007525 | ||
Remark | Similar vulval expression was observed when GFP was fused to the start codon of either egl-18 or elt-6 in a reporter containing ~8 kb of contiguous genomic sequence that includes this ~800 bp region, suggesting that the ~800 bp region is likely to be a vulval enhancer for both genes. As the expression levels and patterns of pKK63 showed substantial variability, even among chromosomal integrants of the transgene, the characterization of the spatial and temporal pattern of egl-18/elt-6::GFP expression is based on the composite pattern that emerged from examination of many animals. | ||
egl-18 = elt-5 | |||
Reference | WBPaper00005609 | ||
Transgene | WBTransgene00027978 |