WormBase Tree Display for Expr_pattern: Expr1639
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| Expr1639 | Expression_of | Gene | WBGene00004854 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004854 | ||
| Expression_data | Life_stage | WBls:0000041 | |
| WBls:0000023 | |||
| WBls:0000018 | |||
| WBls:0000019 | |||
| WBls:0000020 | |||
| WBls:0000017 | |||
| WBls:0000021 | |||
| Anatomy_term (13) | |||
| Type | Reporter_gene | ||
| Pattern | Epidermal cells in C. elegans are born on the dorsal side of the embryo, and the epidermal sheet migrates ventrally to enclose other tissues and anteriorly to cover the head. slt-1::GFP was first detectable just as the migrating epidermal cells completely covered the head and was expressed in hyp4, hyp1, and other epidermal cells. At the 2-fold stage of embryogenesis, expression spread beyond the anterior cap of cells to other anteriorly located cells, including the most anterior head muscle cells and some anterior socket cells, a set of glia associated with head sensilla. During the second half of embryogenesis, when most cells and axons in the developing nervous system are migrating, slt-1::GFP expression was concentrated in anterior cells and excluded from the middle of the body. Expression in anterior cells, including the anterior hyp cells, continued throughout larval stages and in the adult. Around the two-fold stage of embryogenesis, slt-1::GFP expression became evident in pharyngeal cells, including the most posterior pharyngeal muscle. Expression in the pharynx persisted through the L1 stage and in the adult. Beginning at the 2-fold stage of embryogenesis, slt-1::GFP was expressed in dorsal body wall muscles in a striking asymmetric pattern. Dorsal muscle expression began in the posterior embryo and spread anteriorly by the L1 larval stage, all dorsal muscles expressed slt-1::GFP. Robust expression in dorsal muscles persisted throughout the life of the animal. slt-1::GFP was also expressed in ventral body wall muscles, beginning in the L1 stage in posterior ventral muscles and continuing to more anterior muscles in later larval stages. Throughout development, dorsal muscles expressed more slt-1::GFP than ventral muscles. slt-1::GFP was expressed in the BDU neuron in the body and the RIH and RMED neurons, which have the most anterior axons in the nerve ring. | ||
| Expression was first detected in comma stage embryos, with expression restricted to a cap of anterior cells. | |||
| slt-1::GFP is not expressed in ventral epidermal cells. slt-1::GFP was expressed in the anal sphincter muscle. | |||
| Picture | WBPicture0000009612 | ||
| Remark | Double staining with antisera against GFP and the epithelial junction protein JAM-1 (MH27) was performed to characterize embryonic expression in more detail. | ||
| Reference | WBPaper00004900 | ||
| Transgene | WBTransgene00000850 |
