WormBase Tree Display for Expr_pattern: Expr16348
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| Expr16348 | Expression_of | Gene | WBGene00002335 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002335 | ||||
| Expression_data | Life_stage | WBls:0000038 | Anatomy_term | WBbt:0005460 | |
| WBbt:0005784 | |||||
| WBbt:0005813 | |||||
| WBbt:0005828 | |||||
| WBbt:0006749 | |||||
| WBbt:0008373 | |||||
| WBls:0000035 | Anatomy_term | WBbt:0004522 | |||
| WBbt:0005753 | |||||
| WBbt:0005792 | |||||
| WBbt:0007809 | |||||
| WBbt:0008373 | |||||
| WBls:0000041 | Anatomy_term | WBbt:0005319 | |||
| Anatomy_term (11) | |||||
| GO_term | GO:0005730 | ||||
| GO:0005634 | |||||
| GO:0005886 | |||||
| GO:0005829 | |||||
| GO:0012505 | |||||
| Type | Genome_editing | ||||
| Reporter_gene | |||||
| Pattern | mNG::LET-60 was present at high levels in VPCs during VPC induction in the L3 larval stage, where it localized predominantly to the intracellular region-cytosol and possibly endomembranes. We also observed that LET-60 levels were significantly higher in the 1° VPCs compared to the 2° and 3° VPCs during VPC induction where LET-60/Ras activity is higher (Shin & Reiner, 2018). Interestingly, mNG::LET-60 Ras was present at low levels in the uterine anchor cell and during early stages of VPC induction was largely found in the anchor cell nucleus. High levels of mNG::LET-60 were observed in the sex myoblast cells in the intracellular region and nucleus during the L3 and L4 larval stages (Figure 1F), where LET-60/Ras regulates sex myoblast migration and differentiation (Sundaram et al., 1996; Sundaram, 2013). High levels of mNG::LET-60 was also observed in tissues where LET-60/Ras is not known to function, such as nuclear and occasional nucleolar localization of mNG::LET-60 in intestinal cells in early larval stages, regionalized intracellular localization in the pharynx, and high levels of intracellular mNG::LET-60 accumulation in the spermatheca. Enriched intracellular mNG::LET-60 was detected in neurons associated with the nerve ring, consistent with let-60 mRNA expression and LET-60 function in olfactory neurons whose cell bodies and axons are located within the nerve ring (Hirotsu et al., 2000; Menini et al., 2010; Taylor et al., 2021). Finally, plasma membrane localization of mNG::LET-60 was found throughout the germline, in body wall muscle cells, and hypodermal seam cells, which are tissues where LET-60/Ras has known functions (Sundaram, 2013). We noted high levels of intracellular and nuclear mNG::LET-60 in the somatic sheath cells of the gonad, where LET-60 might regulate germline morphogenesis (Lu et al., 2008). | ||||
| Picture | WBPicture0000015233 | ||||
| Reference | WBPaper00065908 | ||||
| Variation | WBVar02158837 |
