WormBase Tree Display for Expr_pattern: Expr14876
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| Expr14876 | Expression_of | Gene | WBGene00001170 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001170 | |||
| Expression_data | Anatomy_term | WBbt:0004926 | Certain | |
| WBbt:0004928 | Certain | |||
| Type | In_situ | smFISH | ||
| Pattern | We used single molecule fluorescence in-situ hybridization (smFISH) to confirm whether endogenous egl-1 mRNA transcripts could be found in URX (JOHNSEN AND HORVITZ 2016). To test the co-localization of the egl-1 fluorescent reporter and endogenous egl-1 mRNA, we used animals that carry the egl-1 reporter transgene nIs343, which expresses nuclear-localized GFP using 6.5 kb of the upstream promoter region of egl-1 (HIROSE AND HORVITZ 2013). These animals showed the same expression pattern as our egl-1 reporter described in Expr14875 (vxIs601), including expression of GFP in a small number of neurons persisting past development, and especially strongly in URX. We found that the GFP expression always (8/8 worms) co-localized with the smFISH signal from probes targeted to the egl-1 transcript. Notably, this expression continued past the L2 larval stage, which is when the last somatic cell deaths in the hermaphrodite occur (SULSTON AND HORVITZ 1977). | |||
| Reference | WBPaper00057346 |
