WormBase Tree Display for Expr_pattern: Expr14305
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| Expr14305 | Expression_of | Gene | WBGene00019883 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00019883 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| WBbt:0007849 | Certain | |||
| WBbt:0007850 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| Type | Antibody | |||
| Pattern | MET-2 was visualized with several reagents, including anti-MET-2 antibody, epitope-tagged transgene generated by mosI-mediated single copy insertion (mosSCI), and endogenously-tagged MET-2 generated by CRISPR-Cas9 editing. These reagents detected nuclear MET-2 throughout the germ line. We observed MET-2 puncta superimposed on a more diffuse signal in germline nuclei and, to a lesser extent, cytoplasm in both male and hermaphrodite germ lines. The MET-2 distribution appeared to shift as germ cells moved from the proliferative region into and through meiosis; nuclear puncta were more obvious in mitotic and leptotene-zygotene nuclei, and the signal became more evenly distributed as nuclei entered and progressed through pachytene stage. We conclude that germline MET-2 comprises nuclear and cytoplasmic pools. | |||
| Reference | WBPaper00056269 | |||
| Antibody_info | WBAntibody00002842 |
