WormBase Tree Display for Expr_pattern: Expr1429
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| Expr1429 | Expression_of | Gene | WBGene00003622 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003622 | |||
| Expression_data | Life_stage | WBls:0000023 | ||
| WBls:0000018 | ||||
| WBls:0000019 | ||||
| WBls:0000020 | ||||
| WBls:0000017 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003681 | Uncertain | ||
| WBbt:0005733 | Uncertain | |||
| WBbt:0005772 | Uncertain | |||
| WBbt:0006281 | Uncertain | |||
| WBbt:0006358 | Uncertain | |||
| Type | Reporter_gene | |||
| Pattern | Epidermal expression of nhr-23 reporter genes can be achieved with as little as 600 bp of sequence upstream of exon I provided that intron I sequences are also included (construct #4281). However, this construct shows additional expression in nuclei of the pharynx and distal 4-6 nuclei of the intestine, two common sites of ectopic expression. | |||
| Expression was completely eliminated by removing intron I (construct #4298) and only ectopic (pharynx and intestine) | ||||
| Expression was observed when mostly intron I sequences (construct #4282) were used to drive expression. | ||||
| Reporter gene constructs that begin at least 1.6 kb upstream of exon I and include all of intron I were consistently expressed in epidermal cells and their precursors. Expression in epidermal cells and precursors was clearly evident by the comma stage of embryogenesis and continued through larval stages. Construct #3306 showed the earliest expression, beginning at about the 50-cell stage in one or two unidentified nuclei. Based on the lineage of epidermal cells, it is possible that these early nuclei are Cpa and Caa; however, authors have been unable to confirm this independently. | ||||
| Picture | WBPicture0000007978 | |||
| Reference | WBPaper00003081 | |||
| Transgene | WBTransgene00027511 |
