WormBase Tree Display for Expr_pattern: Expr13846
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| Expr13846 | Expression_of | Gene | WBGene00013869 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00013869 | |||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
| WBbt:0005319 | Certain | |||
| WBbt:0006760 | Certain | |||
| WBbt:0008217 | Certain | |||
| GO_term | GO:0045178 | |||
| GO:0045177 | ||||
| GO:0005826 | ||||
| Subcellular_localization | During L4, MLCK-1 is localized to apical boundaries of the spermathecal cells, which face the lumen of the spermathecal bag. | |||
| In adults, MLCK-1 is localized both apically and basally in the spermatheca. | ||||
| Type | Genome_editing | |||
| Reporter_gene | CRISPR line COP1510 mlck-1::degron::mKate2 endogenously tagged with GFP strain, was generated by Nemametrix. | |||
| Pattern | the CRISPR line showed MLCK-1 expression in the pharynx, uterus, spermatheca, and sp-ut valve. MLCK-1 is seen throughout the spermatheca, including the distal neck, bag, and valve cells. By mid L4, faint MLCK-1 expression is evident in the cells of the developing spermatheca. Expression levels peak in adulthood. During L4, MLCK-1 is localized to apical boundaries of the spermathecal cells, which face the lumen of the spermathecal bag. In adults, MLCK-1 is localized both apically and basally in the spermatheca. As expected, we observed thick bundles of actin at the outer, basal surface with a thinner actin network visible at the apical surface. MLCK-1 fluorescence intensity is brightest at the apical boundaries. However, some MLCK-1 is also localized to the basal cell surface, where it partially colocalizes with the contractile actomyosin bundles. | |||
| Picture | WBPicture0000014843 | |||
| Reference | WBPaper00054992 |
