WormBase Tree Display for Expr_pattern: Expr1325
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Expr1325 | Expression_of | Gene | WBGene00006771 |
---|---|---|---|
WBGene00003012 | |||
Reflects_endogenous_expression_of | WBGene00003012 | ||
WBGene00006771 | |||
Homol | Homol_homol | CHROMOSOME_II:Expr | |
Expression_data | Life_stage | WBls:0000003 | |
Anatomy_term (7) | |||
GO_term | GO:0005737 | ||
GO:0016328 | |||
Type | Antibody | LIN-26 antibody | |
In_situ | Using antisense probes corresponding to probes used to screen cDNA libraries. | ||
Reporter_gene | |||
Pattern | The GFP::TLN-1 reporter showed prominent expression in all differentiating vulval and uterine cells. In addition to the basal and lateral plasma membrane- associated signal, GFP::TLN-1 expression was also observed in the cytoplasm of the vulval cells of wild-type L3 larvae. | ||
lin-26 transcripts were first weakly detected by in situ hybridization in the germline precursors (P3, P4 and Z2/Z3) until the 50-cell stage and occasionally in their sisters. After the 100-cell stage, lin-26 transcripts and the beta-galactosidase encoded by the lin-26::lacZ construct were detected in hypodermal precursors, then in differentiating hypodermal and support cells. The only difference between in situ hybridization and antibody staining is that lin-26 transcripts could not be detected in body hypodermal cells after the 350-cell stage nor in any cell after the comma stage, whereas LIN-26 protein can be detected until the end of embryogenesis in all hypodermal cells and in all support cells. | |||
Picture | WBPicture0000011694 | ||
WBPicture0000011695 | |||
Remark | Reporter gene fusion type not specified. | ||
Reference | WBPaper00050698 | ||
WBPaper00003532 | |||
Transgene (2) | |||
Antibody_info | WBAntibody00000122 |