WormBase Tree Display for Expr_pattern: Expr1295
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| Expr1295 | Expression_of | Gene | WBGene00001182 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001182 | |||
| Expression_data | Life_stage | WBls:0000038 | ||
| Anatomy_term | WBbt:0005792 | Certain | ||
| WBbt:0006751 | Certain | |||
| WBbt:0006759 | Certain | |||
| WBbt:0006789 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | Translational construct: COG-2::GFP is a nuclear protein. | |||
| Type | Reporter_gene | |||
| Pattern | The cog-2::gfp transcriptional fusion is expressed in a similar pattern to the translational fusion in the pi and pi lineages. The expression pattern of the transcriptional fusion differs from the translational fusion in that the body wall muscles express the fusion protein at a higher level than any other cell and fewer neurons expressing cog-2::gfp can be detected with the transcriptional fusion, although the same brightly expressing cells that stand out with the translational fusion are visible. | |||
| Translational construct: cog-2::gfp is expressed in a dynamic pattern in cells of the pi-lineage. No expression is visible in the gonad until the pi cell precursors are born. At this point, expression is evident in all pi cell precursors as well as in the single pi precursor on each side that has a pi cell precursor as a sister. After the pi cell precursors undergo their single and final division, cog-2::gfp expression is still bright in the pi cells, but expression is usually no longer evident in the lineage. Most animals show no expression of COG-2::GFP expression past the precursor stage in the pi lineage, although in a few animals, faint GFP expression was observed in the daughters, but never the granddaughters, of the expressing pi precursor. cog-2::gfp expression remains strong in the lineage throughout the L4 stage, becoming slightly brighter in the pi cells that adopt the uv1 fate. In addition, the AC nucleus begins to express cog-2::gfp after fusion with the utse cell. Expression in the AC is not detectable before the time of fusion. cog-2::gfp expression is not detectable in the vulva at any stage, in any other part of the gonad including the distal tip cells, or in the hypodermis. cog-2::gfp is expressed in most, if not all, neurons in the head and the tail of the animal. However, four unidentified cells in the head express cog-2::gfp at a higher level than others. Expression is also faintly visible in the body wall muscles and the intestinal cells. | ||||
| Picture | WBPicture0000008004 | |||
| Reference | WBPaper00003386 | |||
| Transgene | WBTransgene00027455 | |||
| WBTransgene00031649 |
